Sister-chromatid exchange in 4 human races.
نویسنده
چکیده
Sister-chromatid exchanges (SCE) are the result of apparent exchanges of DNA between chromatids and may be visualized in metaphase chromosomes after growth of cells for 2 cycles in bromodeoxyuridine and appropriate staining procedures. The frequency of SCEs is thought to be a sensitive indicator of genetic instability and early chromosome changes (Perry and Evans, 1975; Solomon and Bobrow, 1975; Vogel and Bauknecht, 1976; Carrano, 1978).
منابع مشابه
Sister chromatid exchange analysis in some Holstein bulls
Chromosomal appearance of 12 Holstein bulls selected for artificial inseminations were examined bysister chromatid exchanges (SCEs). For differential staining of sister chromatids bromodeoxyuridine (BrdU)was inoculated in lymphocyte cultures. The mean, maximum and minimum number of SCE per cell weredetermined 6.8 ± 1.14, 8.3 ± 1.1 and 5.7 ± 1.5, respectively. SCE frequencies of all animals were...
متن کاملStudy of the Effect of MMC on the Sister Chromatid Exchange in the Human Lymphocytes
Some environmental mutagenic agents cause genomic instability and increase susceptibility of DNA damage. One of them is mitomycin C which is connected to DNA as an alkylating factor and affects susceptible cells to reduction reactions. This drug is used in chemotherapy and treatment of tumors. Study of genomic instability in the presence of different concentrations of MMC can show susceptibilit...
متن کاملEffect of borax on immune cell proliferation and sister chromatid exchange in human chromosomes
BACKGROUND Borax is used as a food additive. It becomes toxic when accumulated in the body. It causes vomiting, fatigue and renal failure. METHODS The heparinized blood samples from 40 healthy men were studied for the impact of borax toxicity on immune cell proliferation (lymphocyte proliferation) and sister chromatid exchange in human chromosomes. The MTT assay and Sister Chromatid Exchange ...
متن کاملMicronucleus and Sister Chromatid Exchange Frequency in Endemic Fluorosis
Inhabitants of the Hohhot Region in Inner Mongolia who drink highfluoride (4-15 mg/L) water were compared for their micronucleus (MN) rate and sister chromatid exchange (SCE) frequency in their peripheral blood lymphocytes. In persons with fluorosis as well as those considered "healthy", the MN rate and SCE frequency were significantly higher (t test) than in a neighbouring control group drinki...
متن کاملAutomatic measurement of sister chromatid exchange frequency.
An automatic system for detecting and counting sister chromatid exchanges in human chromosomes has been developed. Metaphase chromosomes from lymphocytes which had incorporated 5-bromodeoxyuridine for two replication cycles were treated with the dye 33258 Hoechst and photodegraded so that the sister chromatids exhibited differential Giemsa staining. A computer-controlled television-microscope s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Mutation research
دوره 91 4-5 شماره
صفحات -
تاریخ انتشار 1981